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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Immunology | 5–10% | - Serologic procedures and immune diseases - Principles of immunology |
| Topic 2: Laboratory Operations | 5–10% | - Quality assessment and safety - Instrumentation and laboratory mathematics |
| Topic 3: Microbiology | 15–20% | - Bacteriology and mycology procedures - Virology and parasitology testing |
| Topic 4: Hematology | 20–25% | - Laboratory testing and hemostasis - Physiology and disease states |
| Topic 5: Urinalysis and Other Body Fluids | 5–10% | - Microscopic analysis and disease correlation - Physical and chemical testing |
| Topic 6: Chemistry | 20–25% | - Carbohydrates, lipids, and enzymes - Electrolytes, acid-base, and toxicology |
| Topic 7: Blood Banking | 15–20% | - Transfusion practice and testing - Blood products and components - Blood group systems and immunology |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
1. Considering the reactions given in this case study, Yersinia enterocolitica would be the best choice.
Most strains of Plesiomonas shigelloides, Escherichia coli, and Pasteurella multocida are urease negative.
Also, Plesiomonas shigelloides demonstrates a K/A reaction on a TSI slant.
The stool specimen from a patient admitted to the hospital with symptoms of appendicitis has a culture with the following characteristics:
Gram-negative bacillus
Catalase positive
Urease positive
Weakly fermentative
TSI Slant is A/A
These reactions suggest that the organism is MOST likely:
A) Pasteurella multocida
B) Yersinia enterocolitica
C) Plesiomonas shigelloides
D) Escherichia coli
2. PCT usually rises within 3-6 hours of infection. CRP also increases rapidly following infection, but is not as specific for infection as PCT. A rise in CRP could also occur with SIRS. Lactic acid is usually used to detect and monitor impaired circulation and tissue oxygenation in critically ill patients.
Chemistry
Of the three laboratory tests that are listed, which has proven to be most effective for early differentiation of systemic inflammatory response syndrome (SIRS) from sepsis due to its increase following infection and higher specificity?
A) C-reactive protein (CRP)
B) Procalcitonin (PCT)
C) Lactic acid
3. Incompatiblity involving the ABO blood group system can cause the most severe type of transfusion reaction.
The cause of the most severe life-threatening hemolytic transfusion reactions is:
A) Anti-M
B) Anti-A, Anti-B, Anti-A, B
C) Anti-Fya
D) Anti-D
4. Coumarin derivatives inhibit the vitamin K dependent Factors (II, VII, X) which can be measured with the PT and monitored frequently with the INR assay.
Hematology
Warfarin-based (coumarin derivative) oral anti-coagulant therapy is commonly monitored with :
A) APTT and PT
B) PT/INR
C) APTT
D) Thrombin time
5. Serum amylase and lipase levels may be slightly elevated in chronic pancreatitis, but not diagnostic enough to predict chronic pancreatitis; wheras high levels are found only during acute pancreatitis episodes. In the later stages of chronic pancreatitis, normal to decreased levels of amylase and lipase are caused by the gradual inability of the pancreas to secrete the enzyme All of the statements below regarding amylase and lipase in pancreatitis are TRUE EXCEPT:
A) Diagnosis sensitivity is increased by assaying both amylase and lipase.
B) Urinary amylase: creatinine ratio is the most sensitive test for acute pancreatitis.
C) Amylase and lipase are as predictive in chronic as in acute pancreatitis.
D) Serum lipase peaks at 24 hours after an episode of acute pancreatitis and remains high for 7-8 days.
Solutions:
| Question # 1 Answer: B | Question # 2 Answer: B | Question # 3 Answer: B | Question # 4 Answer: D | Question # 5 Answer: C |


